Anti-Raptor抗体(ab125351)
Key features and details
- Rabbit polyclonal to Raptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Raptor抗体
参阅全部 Raptor 一抗 -
描述
兔多克隆抗体to Raptor -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human Raptor aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab155813) -
阳性对照
- This antibody gave a positive signal in the following human whole cell lysates: HepG2; HeLa; HEK293; PANC1; A431.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125351于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/250. Detects a band of approximately 151 kDa (predicted molecular weight: 149 kDa).
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说明 |
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WB
1/250. Detects a band of approximately 151 kDa (predicted molecular weight: 149 kDa). |
靶标
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功能
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. -
组织特异性
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. -
序列相似性
Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats. -
细胞定位
Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability. - Information by UniProt
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数据库链接
- Entrez Gene: 57521 Human
- Omim: 607130 Human
- SwissProt: Q8N122 Human
- Unigene: 133044 Human
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别名
- KIAA1303 antibody
- KOG1 antibody
- Mip1 antibody
see all
图片
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All lanes : Anti-Raptor antibody (ab125351) at 1/250 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 149 kDa
Observed band size: 151 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125351 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125351 尚未被引用在任何文献中。