概述

  • 产品名称Anti-RAPH1抗体
    参阅全部 RAPH1 一抗
  • 描述
    兔多克隆抗体to RAPH1
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to C terminal amino acids 1105-1248 of Human RAPH1.

  • 阳性对照
    • Human duodenum tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6

靶标

  • 功能Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion.
  • 组织特异性Isoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells.
  • 序列相似性Belongs to the MRL family.
    Contains 1 PH domain.
    Contains 1 Ras-associating domain.
  • 细胞定位Cell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9430025M21Rik antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein antibody
    • C730009O10Rik antibody
    • Lamellipodin antibody
    • PREL-2 antibody
    • Proline-rich EVH1 ligand 2 antibody
    • Protein RMO1 antibody
    • RAPH1 antibody
    • RAPH1_HUMAN antibody
    • Ras-associated and pleckstrin homology domains-containing protein 1 antibody
    see all

Anti-RAPH1 antibody 图像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, plasma membrane and cytoplasm. Recommended concentration of ab121619 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121619 at 1/75 staining RALPH1 in Paraffin Embedded Human duodenum tissue. Note strong cytoplasmic positivity in glandular cells.

Anti-RAPH1 antibody (ab121619)参考文献

ab121619 has not yet been referenced specifically in any publications.

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