概述

  • 产品名称Rapamycin
  • 描述
    mTORC1 complex inhibitor
  • 别名
    • Sirolimus
  • 生物学描述mTORC1 complex inhibitor. Binds with FKBP-12 to inhibit mTOR (mammalian target of rapamycin) C1 signaling. Induces autophagy in vitro and in vivo.
  • 纯度> 98%

性能

    应用

    Our Abpromise guarantee covers the use of ab120224 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Rapamycin 图像

    • MCF7 cells were incubated at 37°C for 1h with vehicle control (0 µM) and different concentrations of rapamycin (ab120224). Decreased expression of mTOR (phospho S2448) (ab51044) in MCF7 cells correlates with an increase in rapamycin concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab51044 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    Rapamycin (ab120224)参考文献

    This product has been referenced in:
    • Chen J & Fang Y A novel pathway regulating the mammalian target of rapamycin (mTOR) signaling. Biochem Pharmacol 64:1071-7 (2002). Read more (PubMed: 12234610) »
    • Abraham RT Mammalian target of rapamycin: immunosuppressive drugs uncover a novel pathway of cytokine receptor signaling. Curr Opin Immunol 10:330-6 (1998). Read more (PubMed: 9638370) »
    • Chang JY & Sehgal SN Pharmacology of rapamycin: a new immunosuppressive agent. Br J Rheumatol 30 Suppl 2:62-5 (1991). Read more (PubMed: 1993260) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry.

    The purity and quality of many of our chemical products are determined using a comprehensive range of techniques, including HPLC, chiral HPLC, NMR, microanalysis, optical rotation, TLC, and mass spectrometry. I ca...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"