Anti-RAP2B抗体(ab58109)

概述

  • 产品名称Anti-RAP2B抗体
    参阅全部 RAP2B 一抗
  • 描述
    小鼠单克隆抗体to RAP2B
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-184 of Human RAP2B

性能

应用

Our Abpromise guarantee covers the use of ab58109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.
  • 组织特异性Expressed in red blood cells (at protein level).
  • 序列相似性Belongs to the small GTPase superfamily. Ras family.
  • 结构域The effector domain mediates the interaction with RUNDC3A.
  • 翻译后修饰Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.
  • 细胞定位Recycling endosome membrane. Associated with red blood cells-released vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC20484 antibody
    • RAP 2B antibody
    • RAP2A antibody
    • Rap2b antibody
    • RAP2B member of RAS oncogene family antibody
    • RAP2B_HUMAN antibody
    • Ras family small GTP binding protein RAP2B antibody
    • Ras related protein RAP 2B antibody
    • Ras related protein RAP2B antibody
    • Ras-related protein Rap-2b antibody
    • Small GTP binding protein antibody
    see all

Anti-RAP2B antibody 图像

  • RAP2B antibody (ab58109) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B tissue.


  • Predicted band size : 21 kDa
    RAP2B antibody (ab58109) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • ab58109 at 10 ug/ml staining RAP2B in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing K562 cells stained with ab58109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58109, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-RAP2B antibody (ab58109)参考文献

ab58109 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (RAW 264.7 macrophages cell line)
Loading amount 20 µg
Specification RAW 264.7 macrophages cell line
Gel Running Conditions Reduced Denaturing (4-12% bis-tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
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提交于 Jul 31 2008

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