概述

  • 产品名称Anti-RAP2B抗体
    参阅全部 RAP2B 一抗
  • 描述
    兔多克隆抗体to RAP2B
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 159 of Human RAP2B (AAH12362)

  • 阳性对照
    • A431 whole cell lysate. H1299 and HepG2 cell lines. CL1-0 Xenograft tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab101369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8.0 is recommended

靶标

  • 功能Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.
  • 组织特异性Expressed in red blood cells (at protein level).
  • 序列相似性Belongs to the small GTPase superfamily. Ras family.
  • 结构域The effector domain mediates the interaction with RUNDC3A.
  • 翻译后修饰Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.
  • 细胞定位Recycling endosome membrane. Associated with red blood cells-released vesicles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC20484 antibody
    • RAP 2B antibody
    • RAP2A antibody
    • Rap2b antibody
    • RAP2B member of RAS oncogene family antibody
    • RAP2B_HUMAN antibody
    • Ras family small GTP binding protein RAP2B antibody
    • Ras related protein RAP 2B antibody
    • Ras related protein RAP2B antibody
    • Ras-related protein Rap-2b antibody
    • Small GTP binding protein antibody
    see all

Anti-RAP2B antibody 图像

  • Anti-RAP2B antibody (ab101369) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 21 kDa
  • Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ab101369 at 1/100 dilution.

Anti-RAP2B antibody (ab101369)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

The freezing point of a 20% glycerol solution is about -5C, so freezing at -20C is expected. If you have aliquotted the antibody into small volumes for single experiments, then this will not be a problem. Freezing and thawing can be damaging but not al...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"