RabMAb

Anti-RAP1GAP抗体[Y135] (ab32119)

概述

  • 产品名称Anti-RAP1GAP抗体[Y135]
    参阅全部 RAP1GAP 一抗
  • 描述
    兔单克隆抗体[Y135] to RAP1GAP
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C terminus of human Rap1GAP.

  • 阳性对照
    • WB: Jurkat cell lysate. IHC-P: Human stomach carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 73 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

靶标

  • 功能GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
  • 组织特异性Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
  • 序列相似性Contains 1 GoLoco domain.
    Contains 1 Rap-GAP domain.
  • 细胞定位Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0474 antibody
    • Rap1 GTPase activating protein 1 antibody
    • RAP1 GTPase activating protein antibody
    • Rap1 GTPase-activating protein 1 antibody
    • RAP1GA1 antibody
    • Rap1ga1 protein antibody
    • Rap1GAP antibody
    • Rap1GAP1 antibody
    • RAP1GAPII antibody
    • RAPGAP antibody
    • RPGP1_HUMAN antibody
    see all

Anti-RAP1GAP antibody [Y135] 图像

  • Anti-RAP1GAP antibody [Y135] (ab32119) at 1/10000 dilution + Jurkat cell lysate

    Predicted band size : 73 kDa
    Observed band size : 85-95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Ab32119, at a dilution of 1/100, staining RAP1GA1 in paraffin embedded human stomach carcinoma tissue by immunohistochemistry.

Anti-RAP1GAP antibody [Y135] (ab32119)参考文献

ab32119 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab32119.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"