RabMAb

Anti-RAP1A + RAP1B抗体[EP879Y] (ab75871)

概述

  • 产品名称Anti-RAP1A + RAP1B抗体[EP879Y]
    参阅全部 RAP1A + RAP1B 一抗
  • 描述
    兔单克隆抗体[EP879Y] to RAP1A + RAP1B
  • 经测试应用适用于: WB, IP, ICCmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N terminus of human RAP1A + RAP1B

  • 阳性对照
    • Human brain lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab75871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/40.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    • 相关性RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known.
    • 细胞定位Cell Membrane; Attached to the membrane by a lipid anchor
    • 数据库链接
    • 别名
      • C21KG antibody
      • G 22K antibody
      • GTP binding protein smg p21A antibody
      • GTP binding protein smg p21B antibody
      • K REV antibody
      • KREV 1 antibody
      • KREV antibody
      • KREV1 antibody
      • RAL 1B antibody
      • RAL1B antibody
      • RAP 1 antibody
      • RAP 1A antibody
      • RAP 1B antibody
      • RAP1 antibody
      • RAP1A member of RAS oncogene family antibody
      • RAP1B antibody
      • RAP1B member of RAS oncogene family antibody
      • Ras family small GTP binding protein RAP1B antibody
      • RAS related protein 1b antibody
      • Ras related protein Krev 1 antibody
      • Ras related protein Krev1 antibody
      • Ras related protein RAP 1A antibody
      • Ras related protein RAP 1B antibody
      • RAS related protein RAP1A antibody
      • Ras related protein RAP1B antibody
      • small GTP binding protein antibody
      • SMGP 21 antibody
      • SMGP21 antibody
      see all

    Anti-RAP1A + RAP1B antibody [EP879Y] 图像

    • Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/500 dilution + human brain lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 21 kDa
      Observed band size : 21 kDa

      Secondary antibody - goat anti-rabbit HRP (ab6721)

    • Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/1000 dilution + Mouse brain

      Predicted band size : 21 kDa

      Secondary antibody - goat anti-rabbit HRP (ab6721)

    Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)参考文献

    This product has been referenced in:
    • Todorovic V  et al. Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation. Mol Biol Cell 25:3749-64 (2014). Read more (PubMed: 25208567) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HEPG2)
    Specification HEPG2
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Nov 18 2015

    Application Western blot
    Sample Human Cell lysate - whole cell (HEPG2)
    Gel Running Conditions Reduced Denaturing (12.5)
    Loading amount 20 µg
    Specification HEPG2
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 13 2015

    Application Western blot
    Loading amount 100000 cells
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Specification mouse embryonic stem cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"