Anti-RAP1抗体[78B356] (ab13889)

概述

  • 产品名称Anti-RAP1抗体[78B356]
    参阅全部 RAP1 一抗
  • 描述
    小鼠单克隆抗体[78B356] to RAP1
  • 经测试应用适用于: WBmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    KAEEDPEAADSGEPQNKRTPDLPE

    , corresponding to amino acids 212-235 of Human RAP1.

  • 阳性对照
    • 293 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab13889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 50 kDa.

    Is unsuitable for ICC/IF (please see abreview).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.
    • 组织特异性Ubiquitous. Highly expressed.
    • 序列相似性Belongs to the RAP1 family.
      Contains 1 BRCT domain.
      Contains 1 Myb-like domain.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Cytoplasm. Chromosome. Chromosome > telomere. Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dopamine receptor interacting protein 5 antibody
      • Dopamine receptor-interacting protein 5 antibody
      • DRIP 5 antibody
      • DRIP5 antibody
      • hRap1 antibody
      • MGC105533 antibody
      • RAP 1 antibody
      • RAP1 homolog antibody
      • RAP1, yeast, homolog if antibody
      • RAP1, yeast, homolog of antibody
      • Repressor/activator protein 1 homolog antibody
      • TE2IP_HUMAN antibody
      • Telomeric Repeat Binding Factor 2 Interacting Protein antibody
      • Telomeric repeat-binding factor 2-interacting protein 1 antibody
      • TERF2-interacting protein antibody
      • TERF2-interacting telomeric protein 1 antibody
      • TERF2IP antibody
      • TRF2 Interacting Telomeric Protein RAP1 antibody
      • TRF2 interacting telomeric RAP1 protein antibody
      • TRF2-interacting telomeric protein 1 antibody
      • TRF2-interacting telomeric protein antibody
      see all

    Anti-RAP1 antibody [78B356] 图像

    • Detection of hRap1 in 293 cells: 2 ug/ml of ab13889 were used; a protein band of approximately 50 kDa is detected.

    Anti-RAP1 antibody [78B356] (ab13889)参考文献

    ab13889 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Dec 15 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"