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Our Abpromise guarantee covers the use of ab14404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab91366 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
ab14404 staining RAP1 in the 184A1 epithelial cell line from Human mammary glands by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1), permeabilized with 1% Triton X-100 in PBS and blocked with 1% BSA for 10 minutes at 21°C. Samples were incubated with primary antibody (1/50 in PBS + 1% BSA) for 1 hour at 21°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
ab14404 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"