Anti-RAP1A抗体[1D2-1C64] (ab175329)
Key features and details
- Mouse monoclonal [1D2-1C64] to RAP1A
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
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产品名称
Anti-RAP1A抗体[1D2-1C64]
参阅全部 RAP1A 一抗 -
描述
小鼠单克隆抗体[1D2-1C64] to RAP1A -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow -
免疫原
Recombinant full length protein corresponding to Human RAP1A aa 1 to the C-terminus.
Database link: P62834 -
阳性对照
- HeLa, Hek293T, U2OS and mouse NIH 3T3 cell lysates, HeLa cells, C2C12 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.1% BSA, 69% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1D2-1C64 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175329于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/1000. Predicted molecular weight: 21 kDa.
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IP |
Use at 2 µg/mg of lysate.
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ICC/IF |
1/50 - 1/200.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/1000. Predicted molecular weight: 21 kDa. |
IP
Use at 2 µg/mg of lysate. |
ICC/IF
1/50 - 1/200. |
靶标
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功能
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. -
序列相似性
Belongs to the small GTPase superfamily. Ras family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 282031 Cow
- Entrez Gene: 5906 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- SwissProt: P62833 Cow
- SwissProt: P62834 Human
- SwissProt: P62835 Mouse
see all -
别名
- C21KG antibody
- G 22K antibody
- G-22K antibody
see all
图片
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Immunohistochemistry analysis of RAP1A using ab175329 at 1/200 dilution showing staining in the membrane of paraffin-embedded human kidney tissue (right) compared with a negative control without primary antibody (left). Detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit.
Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
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Immunocytochemical analysis of RAP1A (green) showing staining in the in the cytoplasm of Formalin-fixed and 0.1% Triton X-100 permeabilized C2C12 cells (right) using ab175329 at 1/20 dilution compared to a negative control without primary antibody (left) followed by DyLight-conjugated secondary antibody. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with DAPI.
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All lanes : Anti-RAP1A antibody [1D2-1C64] (ab175329) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : U2OS cell lysate
Lane 4 : Mouse NIH 3T3 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG-HRP at 1/15000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa -
Immunofluorescence analysis of formalin-fixed permeabilized HeLa cells, labeling RAP1A (green, left panel) using ab175329 at a 1/100 dilution followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye (central panel).
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Western blot analysis on immunoprecipitation pellet from mouse NIH 3T3 cells. The antigen-antibody complex was formed by incubating 750 µg of NIH 3T3 whole cell lysate with 2 µg of ab175329 overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose, washes extensively and eluted in sample buffer. 1) 25 µg of NIH 3T3 whole cell lysate, as a control, and 2) eluted sample were resolved on a SDS PAGE gel. The membrane was probed with ab175329 at a 1/500 dilution. Chemiluminescent detection was perfomed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab175329 被引用在 5 文献中.
- Ling Q et al. Anesthetic propofol enhances cisplatin-sensitivity of non-small cell lung cancer cells through N6-methyladenosine-dependently regulating the miR-486-5p/RAP1-NF-κB axis. BMC Cancer 22:765 (2022). PubMed: 35836137
- Yang L et al. TAZ maintains telomere length in TNBC cells by mediating Rad51C expression. Breast Cancer Res 23:89 (2021). PubMed: 34488828
- Ke CC et al. TBC1D21 Potentially Interacts with and Regulates Rap1 during Murine Spermatogenesis. Int J Mol Sci 19:N/A (2018). PubMed: 30360518
- Ling X et al. TERT regulates telomere-related senescence and apoptosis through DNA damage response in male germ cells exposed to BPDE in vitro and to B[a]P in vivo. Environ Pollut 235:836-849 (2018). Mouse . PubMed: 29353801
- Schmölders J et al. Comparative Proteomics of Purified Pathogen Vacuoles Correlates Intracellular Replication of Legionella pneumophila with the Small GTPase Ras-related protein 1 (Rap1). Mol Cell Proteomics 16:622-641 (2017). PubMed: 28183814