Anti-RANTES抗体[VL-1] (ab9666)

概述

  • 产品名称Anti-RANTES抗体[VL-1]
    参阅全部 RANTES 一抗
  • 描述
    小鼠单克隆抗体[VL-1] to RANTES
  • 经测试应用适用于: IHC-P, ELISA, Neutralising, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human).

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab9666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/300. PubMed: 19155524
ELISA 1/8000. Can be paired for ELISA with Rabbit polyclonal to RANTES (ab9679).
Neutralising Use a concentration of 5 - 7 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa.

靶标

  • 功能Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • 组织特异性T-cell and macrophage specific.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta chemokine RANTES antibody
    • Beta chemokine RANTES precursor antibody
    • C C motif chemokine 5 antibody
    • CCL 5 antibody
    • CCL5 antibody
    • CCL5_HUMAN antibody
    • Chemokine (C C motif) ligand 5 antibody
    • Chemokine CC Motif Ligand 5 antibody
    • D17S136E antibody
    • EoCP antibody
    • Eosinophil chemotactic cytokine antibody
    • MGC17164 antibody
    • RANTES(4-68) antibody
    • Regulated upon activation normally T expressed and presumably secreted antibody
    • SCYA 5 antibody
    • SCYA5 antibody
    • SIS delta antibody
    • SIS-delta antibody
    • SISd antibody
    • Small inducible cytokine A5 (RANTES) antibody
    • Small inducible cytokine A5 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
    • Small-inducible cytokine A5 antibody
    • T cell specific protein p288 antibody
    • T cell specific RANTES protein antibody
    • T cell-specific protein P228 antibody
    • T-cell-specific protein RANTES antibody
    • TCP 228 antibody
    • TCP228 antibody
    see all

Anti-RANTES antibody [VL-1] 图像

  • ab9666 staining RANTES in human esophageal squamous cell carcinoma tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blockedwith a blocking agent for 20 minutes at 20°C and no antigen retrieval was conducted. The sample was incubated with primary antibody (1/100 in PBS + 0.1%BSA + 0.2%TritonX-100) at 4°C for 17 hours. A biotin-conjugated horse anti-mouse polyclonal (1/200) was used as the secondary antibody.

Anti-RANTES antibody [VL-1] (ab9666)参考文献

This product has been referenced in:
  • Tan MC  et al. Disruption of CCR5-dependent homing of regulatory T cells inhibits tumor growth in a murine model of pancreatic cancer. J Immunol 182:1746-55 (2009). IHC-P ; Human . Read more (PubMed: 19155524) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (esophageal squamous cell carcinoma)
Antigen retrieval step None - Buffer/Enzyme Used: 10mM citrate, pH6.0
Specification esophageal squamous cell carcinoma
Blocking step Non-Abcam Blocking agent as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative Formaldehyde
Username

Ms. Tatiana Karakasheva

Verified customer

提交于 Feb 22 2016

Application ELISA
Sample Human Cell (tissue culture conditioned media was harvested to)
Specification tissue culture conditioned media was harvested to
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type Sandwich (Capture)
Username

Ms. Tatiana Karakasheva

Verified customer

提交于 Nov 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"