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Our Abpromise guarantee covers the use of ab9679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|ELISA||Use a concentration of 0.5 µg/ml.|
|Neutralising||Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.|
|IHC-P||Use a concentration of 2 µg/ml.|
ab9679 staining RANTES in Human skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 20 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6) and tris buffer (pH 8). Samples were incubated with primary antibody (2μg/ml) for 1 hour at 25°C. An undiluted HRP-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody.
ab9679 staining RANTES in human breast invasive ductal carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 2.0 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with an AEC chromogen.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for RANTES using ab9679 at 1/150 dilution in immunohistochemical analysis. Goat anti-Rabbit HRP was used as the secondary antibody. Heat mediated antigen retrieval using EDTA at pH 9 was used before commencing with the IHC protocol.
Formalin-fixed, paraffin-embedded mouse colon tissue stained for RANTES using ab9679 at 1/150 dilution in immunohistochemical analysis. Goat anti-Rabbit HRP was used as the secondary antibody. Heat mediated antigen retrieval using EDTA at pH 9 was used before commencing with the IHC protocol.