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Bacterially expressed fusion protein, corresponding to amino acids 1-317 of Mouse RANKL
Our Abpromise guarantee covers the use of ab45039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
|IHC-P||Use at an assay dependent concentration.|
Human lymph node probed with ab45039 at 5 µg/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Human liver stained with ab45039 (1:500, 1 µg/ml), peroxidase-conjugate and DAB chromogen. A 2 hr incubation at RT was used.