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Our Abpromise guarantee covers the use of ab13918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 97 kDa.|
|Flow Cyt||Use 5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.
ab13918 has not yet been referenced specifically in any publications.