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Recombinant fragment within Human RANK. The exact sequence is proprietary. Recombinant fragment containing the extracellular domain (amino acids 33-208) of human RANK.
Our Abpromise guarantee covers the use of ab13918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 97 kDa.|
|Flow Cyt||Use 5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Formaldehyde-fixed, paraffin-embedded human skin tissue stained for RANK using ab13918 at 1/50 dilution in immunohistochemical analysis.
Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.