概述
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产品名称RanGAP1 overexpression 293T裂解液(全细胞)
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描述RanGAP1 overexpression裂解液
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常规说明ab94064 is a 293T cell transfected lysate in which Human RanGAP1 has been transiently over-expressed using a pCMV-RanGAP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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经测试应用适用于: WBmore details
性能
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形式Liquid
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存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
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存储溶液pH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... -
纯度Whole Cell Lysate
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研究领域
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背景Function: GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. PTM: Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. Similarity: Belongs to the RNA1 family. Contains 6 LRR (leucine-rich) repeats. Tissue specificity: Highly expressed in brain, thymus and testis.
相关产品
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Corresponding Antibody
应用
Our Abpromise guarantee covers the use of ab94064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. |
RanGAP1 overexpression 293T lysate (whole cell) 图像
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SDS-PAGE - RanGAP1 293T Transfected Lysate - (positive control) (ab94064)ab94064 at 15µg/lane on an SDS-PAGE gel. -
Western blot - RanGAP1 293T Transfected Lysate - (positive control) (ab94064)All lanes : Anti-RanGAP1 antibody (ab88682) at 1/500 dilution
Lane 1 : RanGAP1 293T Transfected Lysate - Positive Control (ab94064)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
non-Abcam, Goat Anti-mouse IgG (H and L) HRP conjugated, at 1/2500 dilution
RanGAP1 overexpression 293T lysate (whole cell) (ab94064)参考文献
ab94064 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"