概述

  • 产品名称Anti-RANBP6抗体
    参阅全部 RANBP6 一抗
  • 描述
    兔多克隆抗体to RANBP6
  • 特异性ab74448 detects endogenous levels of total RANBP6 protein.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human RANBP6.

  • 阳性对照
    • K562, COLO and COS7 cell extracts. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney. IF/ICC: A549 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab74448 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 125 kDa.
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

Anti-RANBP6 antibody 图像

  • ICC/IF image of ab74448 stained A549 cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-RANBP6 antibody (ab74448) at 1/500 dilution

    Lane 1 : K562 cell extracts
    Lane 2 : COLO cell extracts
    Lane 3 : COS7 cell extracts
    Lane 4 : COS7 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 125 kDa
    Observed band size : 125 kDa
  • IHC image of RANBP6 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74448, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

Anti-RANBP6 antibody (ab74448)参考文献

ab74448 has not yet been referenced specifically in any publications.

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