概述

  • 产品名称
  • 描述
    兔多克隆抗体to RanBP2
  • 经测试应用
    适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide (human) corresponding to 3 times repeated sequence in RanBP2 protein:

    SKAPKSGFEGMFTKKE

    , amino acids 1592-1607.

  • 阳性对照
    • HeLa cell lysate and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab64276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 358 kDa (predicted molecular weight: 358 kDa).
IP Use at an assay dependent dilution.
ICC/IF 1/2000.

靶标

  • 功能
    E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
  • 通路
    Protein modification; protein sumoylation.
  • 疾病相关
    Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
  • 序列相似性
    Contains 1 PPIase cyclophilin-type domain.
    Contains 4 RanBD1 domains.
    Contains 8 RanBP2-type zinc fingers.
    Contains 1 TPR repeat.
  • 结构域
    Contains F-X-F-G repeats.
  • 翻译后修饰
    Polyubiquitinated by PARK2, which leads to proteasomal degradation.
  • 细胞定位
    Nucleus > nuclear pore complex. Cytoplasmic filaments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 358 kDa nucleoporin antibody
    • ANE1 antibody
    • E3 SUMO-protein ligase RanBP2 antibody
    • IIAE3 antibody
    • Nuclear pore complex protein Nup358 antibody
    • Nucleoporin 358 antibody
    • Nucleoporin Nup358 antibody
    • NUP358 antibody
    • p270 antibody
    • RAN binding protein 2 antibody
    • Ran-binding protein 2 antibody
    • RANBP2 antibody
    • RBP2_HUMAN antibody
    • Transformation related protein 2 antibody
    • TRP 1 antibody
    • TRP 2 antibody
    • TRP1 antibody
    • TRP2 antibody
    see all

图片

  • Anti-RanBP2 antibody (ab64276) at 1/1000 dilution + HeLa total cell lysate

    Predicted band size : 358 kDa
    Observed band size : 358 kDa
  • ab64276 (1:2000) staining RanBP2 in Hela cells pre-extracted in 0.005% digitonin and fixed in 3.7% PFA/30 mM sucrose/PBS for 15 min (room temperature). Image kindly provided by Patrizia Lavia, Univ. "La Sapienza", CNR, Italy.
  • RanBP2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RanBP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64276.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .
    Band: 358kDa: RanBP2.
  • Immunofluorescent staining of paraformaldehyde fixed HeLa cells by ab64276 (1:2000).
  • Anti-RanBP2 antibody (ab64276) at 1/2000 dilution + Hela whole cell lysate

    Predicted band size : 358 kDa


    Exposure time : 1 minuteWB performed on a 6%SDS-PAGE gel.

    Image Kindly provided by Patrizia Lavia, University "La Sapienza", CNR, Italy.

文献

This product has been referenced in:
  • Mamede JI  et al. Cyclophilins and nucleoporins are required for infection mediated by capsids from circulating HIV-2 primary isolates. Sci Rep 7:45214 (2017). WB ; Human . Read more (PubMed: 28345672) »
  • Dharan A  et al. KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection. PLoS Pathog 12:e1005700 (2016). Read more (PubMed: 27327622) »

See all 11 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 26 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - 0.5% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 15 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Sample
Human Cell lysate - whole cell (NALM6)
Specification
NALM6
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

提交于 May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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