Anti-RanBP2抗体(ab112061)
Key features and details
- Rabbit polyclonal to RanBP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RanBP2抗体
参阅全部 RanBP2 一抗 -
描述
兔多克隆抗体to RanBP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1050-1100 of Human RanBP2 (NP_006258.3).
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阳性对照
- HeLa Whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab112061 was affinity purified using an epitope specific to RanBP2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112061于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 358 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 358 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. -
通路
Protein modification; protein sumoylation. -
疾病相关
Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem. -
序列相似性
Contains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
结构域
Contains F-X-F-G repeats. -
翻译后修饰
Polyubiquitinated by PARK2, which leads to proteasomal degradation. -
细胞定位
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 5903 Human
- Omim: 601181 Human
- SwissProt: P49792 Human
- Unigene: 199561 Human
- Unigene: 715056 Human
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别名
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
图片
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All lanes : Anti-RanBP2 antibody (ab112061) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 358 kDa
Exposure time: 10 seconds -
Detection of RanBP2 by Western Blot of Immunprecipitate.
ab112061 at 0.1µg/ml staining RanBP2 in HeLa whole cell lysate immunoprecipitated using ab112061 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
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Datasheet download
文献 (2)
ab112061 被引用在 2 文献中.
- Yatskevich S et al. Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell Rep 34:108929 (2021). PubMed: 33789095
- Zhu T et al. Neddylation controls basal MKK7 kinase activity in breast cancer cells. Oncogene 35:2624-33 (2016). PubMed: 26364603