概述

  • 产品名称Anti-RanBP1抗体
    参阅全部 RanBP1 一抗
  • 描述
    兔多克隆抗体to RanBP1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding a region between residues 1 and 50 of human RanBP1 (NP_002873.1)

  • 阳性对照
    • HeLa whole cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab70185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
    • 序列相似性Belongs to the RANBP1 family.
      Contains 1 RanBD1 domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HTF9A antibody
      • RAN binding protein 1 antibody
      • Ran specific GTPase activating protein antibody
      • Ran-binding protein 1 antibody
      • Ran-specific GTPase-activating protein antibody
      • RANBP1 antibody
      • RANG_HUMAN antibody
      see all

    Anti-RanBP1 antibody 图像

    • Lane 1 : Anti-RanBP1 antibody (ab70185) at 1 µg/ml
      Lane 2 : Anti-RanBP1 antibody (ab70185) at 0.33 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 23 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes
    • Western Blot showing ab70185 at 1µg/ml staining RanBP1 in HeLa whole cell lysates immunoprecipitated using ab70185 at 5µg/0.5mg lysate (Lane 1). Lane 2: Control IgG was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 5 minutes

    Anti-RanBP1 antibody (ab70185)参考文献

    ab70185 has not yet been referenced specifically in any publications.

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