Anti-RanBP1抗体(ab70185)
Key features and details
- Rabbit polyclonal to RanBP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RanBP1抗体
参阅全部 RanBP1 一抗 -
描述
兔多克隆抗体to RanBP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding a region between residues 1 and 50 of human RanBP1 (NP_002873.1)
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阳性对照
- HeLa whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab70185 was affinity purified using an epitope specific to RanBP1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70185于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | ||
IP |
说明 |
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WB: 1/1000 - 1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane. -
序列相似性
Belongs to the RANBP1 family.
Contains 1 RanBD1 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5902 Human
- Omim: 601180 Human
- SwissProt: P43487 Human
- Unigene: 24763 Human
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别名
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
see all
图片
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Lane 1 : Anti-RanBP1 antibody (ab70185) at 1 µg/ml
Lane 2 : Anti-RanBP1 antibody (ab70185) at 0.33 µg/ml
All lanes : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes -
Western Blot showing ab70185 at 1µg/ml staining RanBP1 in HeLa whole cell lysates immunoprecipitated using ab70185 at 5µg/0.5mg lysate (Lane 1). Lane 2: Control IgG was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 5 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab70185 尚未被引用在任何文献中。