概述

  • 产品名称
  • 描述
    兔多克隆抗体to RanBP1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Synthetic peptide corresponding to Human RanBP1 aa 1-18.
    Sequence:

    MAAAKDTHEDHDTSTENT


    (Peptide available as ab4938)

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab2937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).Can be blocked with RanBP1 peptide (ab4938).

靶标

图片

  • All lanes : Anti-RanBP1 antibody (ab2937) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : NIH-3T3 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP conjugated anti-rabbit
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

实验方案

文献

This product has been referenced in:
  • Prpar Mihevc S  et al. TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. Sci Rep 6:33996 (2016). WB, ICC/IF . Read more (PubMed: 27665936) »
  • Štalekar M  et al. Proteomic analyses reveal that loss of TDP-43 affects RNA processing and intracellular transport. Neuroscience 293:157-70 (2015). WB, ICC/IF . Read more (PubMed: 25743254) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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