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Our Abpromise guarantee covers the use of ab17034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).Can be blocked with Human RanBP1 peptide (ab23305).|
ab17034 (3.8µg/ml) staining of paraffin embedded Human Kidney tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows pixulated cytoplasm staining in DCT.
Primary Incubation 1 hour.
ab17034 has not yet been referenced specifically in any publications.