概述

  • 产品名称
    Anti-RanBP1抗体
    参阅全部 RanBP1 一抗
  • 描述
    山羊多克隆抗体to RanBP1
  • 宿主
    Goat
  • 经测试应用
    适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: LSVKEETKEDAEEKQ, corresponding to amino acids 187-201 of Human RanBP1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • HeLa and HepG2 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab17034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).Can be blocked with Human RanBP1 peptide (ab23305).
ELISA 1/16000.

靶标

  • 功能
    Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
  • 序列相似性
    Belongs to the RANBP1 family.
    Contains 1 RanBD1 domain.
  • Information by UniProt
  • 数据库链接
    • 别名
      • HTF9A antibody
      • RAN binding protein 1 antibody
      • Ran specific GTPase activating protein antibody
      • Ran-binding protein 1 antibody
      • Ran-specific GTPase-activating protein antibody
      • RANBP1 antibody
      • RANG_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP1 antibody (ab17034)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP1 antibody (ab17034)

      ab17034 (3.8µg/ml) staining of paraffin embedded Human Kidney tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows pixulated cytoplasm staining in DCT.

    • Western blot - Anti-RanBP1 antibody (ab17034)
      Western blot - Anti-RanBP1 antibody (ab17034)
      Anti-RanBP1 antibody (ab17034) at 0.05 µg/ml + HeLa cell Lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 23 kDa



      Primary Incubation 1 hour.

    • Western blot - Anti-RanBP1 antibody (ab17034)
      Western blot - Anti-RanBP1 antibody (ab17034)
      ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence. ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.

    文献

    ab17034 has not yet been referenced specifically in any publications.

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