Anti-Ran抗体, prediluted (ab31120)

概述

  • 产品名称Anti-Ran抗体, prediluted
    参阅全部 Ran 一抗
  • 描述
    兔多克隆抗体to Ran, prediluted
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    EHDLEVAQTTALPDEDDDL

    , corresponding to amino acids 197-216 of Human Ran

  • 阳性对照
    • HeLa and A431 cells. Normal breast

性能

应用

Our Abpromise guarantee covers the use of ab31120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
ICC/IF
WB
  • 应用说明IHC-P: Ready to use for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
    ICC/IF: Use undiluted. Please refer to ICC/IF image below for further details.
    WB: 1/10 - 1/30. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa). Please refer to WB image below for further details.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
      Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
    • 组织特异性Expressed in a variety of tissues.
    • 序列相似性Belongs to the small GTPase superfamily. Ran family.
    • 翻译后修饰The N-terminus is blocked.
    • 细胞定位Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Androgen receptor associated protein 24 antibody
      • Androgen receptor-associated protein 24 antibody
      • ARA 24 antibody
      • ARA24 antibody
      • Gsp1 antibody
      • GTP binding nuclear protein RAN antibody
      • GTP-binding nuclear protein Ran antibody
      • GTPase Ran antibody
      • Guanosine triphosphatase Ran antibody
      • LPS antibody
      • OK/SW-cl.81 antibody
      • ran antibody
      • RAN member RAS oncogene family antibody
      • RAN_HUMAN antibody
      • RanGTPase antibody
      • Ras like protein TC4 antibody
      • Ras related nuclear protein antibody
      • Ras-like protein TC4 antibody
      • Ras-related nuclear protein antibody
      • RASL2 8 antibody
      • TC 4 antibody
      • TC4 antibody
      see all

    Anti-Ran antibody, prediluted 图像

    • Human breast stained with ab31120 for 30 minutes at room temperature.
    • ab31120 staining Ran in HeLa and mouse NIH-3T3 cells, by immunofluorescence.This antibody interacts specifically well with the spindle pole-associated fraction of Ran in mitosis (see image above) and may be extremely useful in studies of mitotic functions of Ran.

      Experimental conditions:

      ab31120 staining Ran in HeLa and mouse NIH-3T3 cells, by immunofluorescence.This antibody interacts specifically well with the spindle pole-associated fraction of Ran in mitosis (see image above) and may be extremely useful in studies of mitotic functions of Ran.

      Experimental conditions:

      optimal antibody dilution: use undiluted.

      optimal fixation protocol:  PFA fixation:10 min in 3,7 % PFA  at room temperature, 5 min in PBS/ 0,1%Triton X-100,  then 3 washes in PBS.

      IF staining was performed following a standard protocol: blocking: 30 min; primary antibody: 1 hr; secondary antibody : 45 min. All incubations were at 37 °C in PBS/ 0,1% Tween containing 3% BSA.



    • Predicted band size : 26 kDa

      Image and experimental conditions kindly submitted by Emanuele Roscioli and Patrizia Lavia, from the University 'La Sapienza' CNR, Italy

      Ab31120 staining Ran in Hela and NIH-3T3 cells, by western blotting. This antibody worked very well with both protein extracts, although some non-specific bands were occasionally seen using concentrated  antibody.

      Optimal antibody dilution: 1/30

      WB was performed following a standard protocol: blocking: 30 min; primary antibody: 1 hr; secondary antibody: 30 min; and developed with ECL Plus (Amersham).

      Anti-Ran antibody, prediluted (ab31120)参考文献

      ab31120 has not yet been referenced specifically in any publications.

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