The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23308199
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Use at an assay dependent concentration.
功能GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
组织特异性Expressed in a variety of tissues.
序列相似性Belongs to the small GTPase superfamily. Ran family.
翻译后修饰The N-terminus is blocked.
细胞定位Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
All lanes : Anti-Ran antibody (ab53775) at 1/500 dilution
Lane 1 : Extracts from LOVO cells Lane 2 : Extracts from LOVO cells, plus immunising peptide
Predicted band size : 26 kDa Observed band size : 26 kDa
Immunohistochemistry (Paraffin-embedded sections) - Ran antibody (ab53775)
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using ab53775 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
Anti-Ran antibody (ab53775)参考文献
This product has been referenced in:
Mastroeni D et al. Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology. PLoS One8:e53349 (2013).
WB, IHC-P, ICC/IF
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