The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
功能GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
组织特异性Expressed in a variety of tissues.
序列相似性Belongs to the small GTPase superfamily. Ran family.
翻译后修饰The N-terminus is blocked.
细胞定位Nucleus. Cytoplasm. Melanosome. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.