概述

  • 产品名称Anti-RAMP3抗体
    参阅全部 RAMP3 一抗
  • 描述
    兔多克隆抗体to RAMP3
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    A synthetic peptide corresponding to N-terminal residues of human RAMP3

性能

应用

Our Abpromise guarantee covers the use of ab58660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性RAMP3 (receptor calcitonin activity modifying protein 3) transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. It acts as a receptor for adrenomedullin (AM) together with CALCRL. It is strongly expressed in lung, breast, immune system and fetal tissues and belongs to the RAMP family.
  • 细胞定位Cell Membrane; Single-pass type I membrane protein
  • 数据库链接
  • 别名
    • Calcitonin receptor like receptor activity modifying protein 3 antibody
    • CRLR activity modifying protein 3 antibody
    • CRLR activity-modifying protein 3 antibody
    • OTTHUMP00000159549 antibody
    • OTTHUMP00000214279 antibody
    • OTTHUMP00000214280 antibody
    • Receptor (calcitonin) activity modifying protein 3 antibody
    • Receptor (G protein-coupled) activity modifying protein 3 antibody
    • Receptor activity modifying protein 3 antibody
    see all

Anti-RAMP3 antibody 图像

  • ICC/IF image of ab58660 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58660, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RAMP3 antibody (ab58660)参考文献

This product has been referenced in:
  • Brekhman V  et al. Receptor activity modifying protein-3 mediates the protumorigenic activity of lysyl oxidase-like protein-2. FASEB J : (2010). Read more (PubMed: 20802105) »

See 1 Publication for this product

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