概述

  • 产品名称
    Anti-RALY抗体
    参阅全部 RALY 一抗
  • 描述
    小鼠多克隆抗体to RALY
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human RALY (AAI03754.1).

  • 阳性对照
    • HepG2 cell lysate; RALY transfected 293T cell lysate and HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab88616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing.
  • 组织特异性
    Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.
  • 序列相似性
    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Autoantigen p542 antibody
      • Heterogeneous nuclear ribonucleoprotein C-like 2 antibody
      • hnRNP associated with lethal yellow protein homolog antibody
      • hnRNP core protein C-like 2 antibody
      • MGC117312 antibody
      • P542 antibody
      • RALY antibody
      • RALY_HUMAN antibody
      • RNA binding protein (autoantigenic hnRNP associated with lethal yellow) antibody
      • RNA binding protein (autoantigenic) antibody
      • RNA binding protein autoantigenic (hnRNP associated with lethal yellow homolog (mouse)) antibody
      • RNA-binding protein Raly antibody
      see all

    图片

    • Western blot - Anti-RALY antibody (ab88616)
      Western blot - Anti-RALY antibody (ab88616)
      Anti-RALY antibody (ab88616) at 1 µg/ml + HepG2 cell lysate at 50 µg

      Predicted band size: 32 kDa
      Observed band size: 32 kDa

    • Western blot - Anti-RALY antibody (ab88616)
      Western blot - Anti-RALY antibody (ab88616)
      All lanes : Anti-RALY antibody (ab88616) at 1 µg/ml

      Lane 1 : RALY transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 32 kDa
      Observed band size: 36 kDa (why is the actual band size different from the predicted?)

    • Immunocytochemistry/ Immunofluorescence - Anti-RALY antibody (ab88616)
      Immunocytochemistry/ Immunofluorescence - Anti-RALY antibody (ab88616)
      ab88616 at 10µg/ml staining RALY in HeLa cells by Immunofluorescence.

    文献

    ab88616 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab88616？请让我们知道，以便我们可以引用本数据表中的参考文章。

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