概述

  • 产品名称Anti-RALY抗体
    参阅全部 RALY 一抗
  • 描述
    兔多克隆抗体to RALY
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • 免疫原

    A region within synthetic peptide: QENTTSEAGL PQGEARTRDD GDEEGLLTHS EEELEHSQDT DADDGALQ, corresponding to C terminal amino acids 243-290 of Human RALY

  • 阳性对照
    • Jurkat cell lysate. Human muscle tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab66263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing.
  • 组织特异性Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.
  • 序列相似性Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Autoantigen p542 antibody
    • Heterogeneous nuclear ribonucleoprotein C-like 2 antibody
    • hnRNP associated with lethal yellow protein homolog antibody
    • hnRNP core protein C-like 2 antibody
    • MGC117312 antibody
    • P542 antibody
    • RALY antibody
    • RALY_HUMAN antibody
    • RNA binding protein (autoantigenic hnRNP associated with lethal yellow) antibody
    • RNA binding protein (autoantigenic) antibody
    • RNA binding protein autoantigenic (hnRNP associated with lethal yellow homolog (mouse)) antibody
    • RNA-binding protein Raly antibody
    see all

Anti-RALY antibody 图像

  • Anti-RALY antibody (ab66263) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labeling RALY with ab66263 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

Anti-RALY antibody (ab66263)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab66263.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"