概述

  • 产品名称Anti-RALGDS抗体
    参阅全部 RALGDS 一抗
  • 描述
    兔多克隆抗体to RALGDS
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human RALGDS.

    (Peptide available as ab75088.)

  • 阳性对照
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab65204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-RALGDS antibody 图像

  • Anti-RALGDS antibody (ab65204) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 100 kDa


    Exposure time : 3 minutes
  • ICC/IF image of ab65204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of RALGDS staining in Human Liver Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65204, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-RALGDS antibody (ab65204)参考文献

ab65204 has not yet been referenced specifically in any publications.

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