概述

  • 产品名称Anti-RALBP1抗体
    参阅全部 RALBP1 一抗
  • 描述
    小鼠单克隆抗体to RALBP1
  • 经测试应用适用于: WB, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-656 of Human RALBP1

性能

应用

Our Abpromise guarantee covers the use of ab56815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 76 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
  • 组织特异性Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
  • 序列相似性Contains 1 Rho-GAP domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • RLIP1 antibody
    • 76 kDa Ral-interacting protein antibody
    • 76-kDa Ral-interacting protein antibody
    • Dinitrophenyl S-glutathione ATPase antibody
    • DNP-SG ATPase antibody
    • Ral-interacting protein 1 antibody
    • Ral-interacting protein 1, 76-KD antibody
    • RalA-binding protein 1 antibody
    • RalBP1 antibody
    • RBP1_HUMAN antibody
    • RIP1 antibody
    • RLIP1 antibody
    • RLIP76 antibody
    see all

Anti-RALBP1 antibody 图像



  • Predicted band size : 76 kDa
  • Overlay histogram showing HEK293 cells stained with ab56815 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56815, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • IHC image of ab56815 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56815, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-RALBP1 antibody (ab56815) at 1/1000 dilution + HCT116 cell lysate at 30 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-RALBP1 antibody (ab56815)参考文献

This product has been referenced in:
  • Zhou B  et al. Knockdown of CDC2 expression inhibits proliferation, enhances apoptosis, and increases chemosensitivity to temozolomide in glioblastoma cells. Med Oncol 32:378 (2015). Read more (PubMed: 25433945) »
  • Wang Q  et al. RLIP76 is overexpressed in human glioblastomas and is required for proliferation, tumorigenesis and suppression of apoptosis. Carcinogenesis 34:916-26 (2013). Human . Read more (PubMed: 23276796) »

See all 4 Publications for this product

Product Wall


Unfortunately the epitope of ab56815 has not been determined and therefore I cannot comment on whether this antibody is likey to recognise your protein fragment.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 30 µg
Specification HCT116
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Nov 30 2012

Thank you for confirming these details and for your cooperation.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement:
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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of prod...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293,HELA and JURKAT)
Loading amount 10 µg
Specification HEK293,HELA and JURKAT
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 02 2011

Thank you for your enquiry. Your search using the keyword "RLIP76" pulled out two RIP1 antibody datasheets (ab33446, ab56815). Although the product name is different, these products are RLIP76 antibodies. If you click on the [Alternative names] lin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"