Anti-RALBP1抗体(ab33446)

Abreviews (1)Q&A (2)

概述

  • 产品名称
    Anti-RALBP1抗体
    参阅全部 RALBP1 一抗
  • 描述
    兔多克隆抗体to RALBP1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human RALBP1.

    参阅Abcam的专有抗源政策

    (Peptide available as ab33445.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat, HEK293, Hela and A431. The data for Hela and A431 is not shown.

性能

应用

Our Abpromise guarantee covers the use of ab33446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).

靶标

  • 功能
    Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
  • 组织特异性
    Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
  • 序列相似性
    Contains 1 Rho-GAP domain.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • RLIP1 antibody
      • 76 kDa Ral-interacting protein antibody
      • 76-kDa Ral-interacting protein antibody
      • Dinitrophenyl S-glutathione ATPase antibody
      • DNP-SG ATPase antibody
      • Ral-interacting protein 1 antibody
      • Ral-interacting protein 1, 76-KD antibody
      • RalA-binding protein 1 antibody
      • RalBP1 antibody
      • RBP1_HUMAN antibody
      • RIP1 antibody
      • RLIP1 antibody
      • RLIP76 antibody
      see all

    图片

    • Western blot - Anti-RALBP1 antibody (ab33446)
      Western blot - Anti-RALBP1 antibody (ab33446)
      All lanes : Anti-RALBP1 antibody (ab33446) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 76 kDa
      Observed band size: 90 kDa (why is the actual band size different from the predicted?)



      The band seen at 90 kDa is consistent with the banding pattern observed for other commercially available antibodies to RIP1.
    • Immunocytochemistry/ Immunofluorescence - RIP1 antibody (ab33446)
      Immunocytochemistry/ Immunofluorescence - RIP1 antibody (ab33446)
      ICC/IF image of ab33446 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33446, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    文献

    ab33446 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab33446？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    3 reviews or Q&A

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    Western blot abreview for Anti-RALBP1 antibody

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    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    20 µg
    Specification
    HEK293
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    Username

    Abcam user community

    Verified customer

    提交于 Feb 01 2013

    Dear Technician,

    I bought Anti-PARP antibody (ab110915), Anti-Calpain 1 antibody (ab3589), Anti-RIP3 antibody (ab16090), Anti-RIP1 antibody (ab33446) from abcam. I need to have the positive control for doing my western blot experiment. Would y...

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    Thank you for contacting us. We do have positive controls available for these antibodies. The Jurkat lysate catalog number is ab7899. This should also be an appropriate control for the anti-RIP3 antibody, as we do not have the HEK293 transfected cells ...

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    I was looking for an antibody to RLIP76 so I typed that into the search box. When I did so, the search results included RLIP76 and it correctly described a DNP-SG ATPase. However, when I clicked on it, it fed me to some other protein called RIP1 (ab334...

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    Thank you for your enquiry. Your search using the keyword "RLIP76" pulled out two RIP1 antibody datasheets (ab33446, ab56815). Although the product name is different, these products are RLIP76 antibodies. If you click on the [Alternative names] lin...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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