The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC/IF: Use at a concentration of 5 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 22 kDa. Can be blocked with RAIDD peptide (99/117) (ab8460) by incubating peptide and antibody, in equal volumes, for 30 minutes at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
组织特异性Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
序列相似性Contains 1 CARD domain. Contains 1 death domain.
结构域Contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2.