The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 20738874
Use a concentration of 1 µg/ml. Predicted molecular weight: 203 kDa.
Use at an assay dependent dilution.
RAI1 (retinoid-acid induced protein 1) may be involved in neuronal differentiation. RAI1 is highly similar to its mouse counterpart and is expressed at high levels mainly in neuronal tissues. RAI1 has a polymorphic polyglutamine tract in it's N-terminal domain. Expression of the mouse counterpart in neurons is induced by retinoic acid. The RAI1 gene is associated with both the severity of the phenotype and the response to medication in schizophrenic patients. Defects in RAI1 are a cause of Smith-Magenis syndrome (SMS). There are four named isoforms.
Cytoplasmic and Nuclear. In neurons it is localized to neurites.
Immunocytochemistry/ Immunofluorescence - Anti-RAI1 antibody (ab58658)Image from Carmona-Mora P et al., BMC Mol Biol. 2010 Aug 25;11:63. Fig 4.; doi:10.1186/1471-2199-11-63; 25 August 2010
ab58658 staining RAI1 (green) in murine N2a cells transfected with RAI1-HA wild type and mutant forms, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200). An AlexaFluor®488-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
Fragoso YD et al. Expression in the human brain of retinoic acid induced 1, a protein associated with neurobehavioural disorders. Brain Struct Funct220:1195-203 (2015).
Read more (PubMed: 24519454) »
Carmona-Mora P et al. Functional and cellular characterization of human Retinoic Acid Induced 1 (RAI1) mutations associated with Smith-Magenis Syndrome. BMC Mol Biol11:63 (2010).
Read more (PubMed: 20738874) »