概述

  • 产品名称
    Anti-RAI1抗体
    参阅全部 RAI1 一抗
  • 描述
    兔多克隆抗体to RAI1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to N terminal residues of Human RAI1

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 20738874
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 203 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性
    RAI1 (retinoid-acid induced protein 1) may be involved in neuronal differentiation. RAI1 is highly similar to its mouse counterpart and is expressed at high levels mainly in neuronal tissues. RAI1 has a polymorphic polyglutamine tract in it's N-terminal domain. Expression of the mouse counterpart in neurons is induced by retinoic acid. The RAI1 gene is associated with both the severity of the phenotype and the response to medication in schizophrenic patients. Defects in RAI1 are a cause of Smith-Magenis syndrome (SMS). There are four named isoforms.
  • 细胞定位
    Cytoplasmic and Nuclear. In neurons it is localized to neurites.
  • 数据库链接
    • 别名
      • DKFZP434A139 antibody
      • KIAA1820 antibody
      • MGC12824 antibody
      • retinoic acid induced 1 antibody
      • Retinoid acid induced protein 1 antibody
      • SMCR antibody
      • SMS antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-RAI1 antibody (ab58658)
      Immunocytochemistry/ Immunofluorescence - Anti-RAI1 antibody (ab58658)Image from Carmona-Mora P et al., BMC Mol Biol. 2010 Aug 25;11:63. Fig 4.; doi:10.1186/1471-2199-11-63; 25 August 2010
      ab58658 staining RAI1 (green) in murine N2a cells transfected with RAI1-HA wild type and mutant forms, by Immunocytochemistry/ Immunofluorescence.

      Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200). An AlexaFluor®488-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    文献

    This product has been referenced in:
    • Fragoso YD  et al. Expression in the human brain of retinoic acid induced 1, a protein associated with neurobehavioural disorders. Brain Struct Funct 220:1195-203 (2015). WB, IHC-P ; Human . Read more (PubMed: 24519454) »
    • Carmona-Mora P  et al. Functional and cellular characterization of human Retinoic Acid Induced 1 (RAI1) mutations associated with Smith-Magenis Syndrome. BMC Mol Biol 11:63 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20738874) »

    See all 2 Publications for this product

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