Anti-RAGE抗体[MM0520-8D11] (ab89911)

概述

  • 产品名称
    Anti-RAGE抗体[MM0520-8D11]
    参阅全部 RAGE 一抗
  • 描述
    小鼠单克隆抗体[MM0520-8D11] to RAGE
  • 宿主
    Mouse
  • 经测试应用
    适用于: Flow Cyt, WB, Neutralisingmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human RAGE extracellular domain.

性能

应用

Our Abpromise guarantee covers the use of ab89911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 24567328

ab18428 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.
Neutralising Use at an assay dependent concentration.

靶标

  • 功能
    Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • 组织特异性
    Endothelial cells.
  • 序列相似性
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Advanced glycosylation end product-specific receptor antibody
    • Ager antibody
    • MGC2235 antibody
    • RAGE_HUMAN antibody
    • Receptor for advanced glycosylation end products antibody
    see all

图片

  • Flow Cytometry analysis of human mesenchymal stem cells labelling RAGE with ab89911. A Phycoerythrin-conjugated F(ab′)2 goat anti-mouse IgG was used as secondary antibody. Histograms were set according to negative control stainings (#).

文献

This product has been referenced in:
  • Chen YH  et al. AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway. J Diabetes Res 2018:6823058 (2018). Read more (PubMed: 29744367) »
  • Vogel S  et al. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET. J Biol Chem 289:11068-82 (2014). Flow Cyt ; Human . Read more (PubMed: 24567328) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your phone call. I'd be happy to issue you either a credit or refund. Alternatively, a free of charge replacement with another antibody can be sent. I have checked our catalog for other potential suitable RAGE antibodies to ...

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Thank you for your patience. The lab send the follwoing information and suggestion: (1) The immunogen of this antibody is the extracellular domain (based on the UniProt database entry Q15109: residues 24 to 344). (2) The customer...

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Thank you for contacting us. I have passed on your experimental details to the lab for more input, and will let you know what they suggest. Regarding your questions: 1) Which part of the epitope is this antibody raised against? ...

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Thank you for contacting us. Here is the reply I received from the lab. First to answer your question about the testing protocol used by the lab for neutralization studies: The neutralization function of this antibody was tested ...

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Thanks for your inquiry. Our neutralization assay was done in vitro between RAGE and AGE-BAS. It means the antibody indeed blocks the binding of the receptor from AGE in the in vitro setting. I hope this answers your question. Plea...

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Thank you for your calls yesterday, and for your patience. According to the lab, the antibody was tested in vitro using a functional ELISA as follows:   If uninhibited, biotinylated AGE-BSA can bind to immobilized recombinant human RAGE coated on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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