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Our Abpromise guarantee covers the use of ab7764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution. PubMed: 16941014See Abreviews. Suggested working dilution of 1/400 from PMID 16941014.|
ab7764 staining RAGE in mouse lung tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before antigen retrieval which involved boiling citric acid and then blocking at 370C for 15 minutes was performed. The primary antibody was diluted in 1/250 and it was incubated with sample for 1 hour at 37°C. A HRP conjugated rat polyclonal to goat IgG was used undiluted as a secondary antibody.