概述

性能

应用

Our Abpromise guarantee covers the use of ab37647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Flow Cyt Use 1µg for 106 cells. Use PBS/EDTA to detach cells to preserve the glycoproteins on the cell surface; do not fix, do not permeabilise.



ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ELISA 1/2000 - 1/10000.

靶标

  • 功能Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • 组织特异性Endothelial cells.
  • 序列相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Advanced glycosylation end product-specific receptor antibody
    • Ager antibody
    • MGC2235 antibody
    • RAGE_HUMAN antibody
    • Receptor for advanced glycosylation end products antibody
    see all

Anti-RAGE antibody 图像

  • All lanes : Anti-RAGE antibody (ab37647) at 1/2000 dilution

    Lane 1 : Bovine lung extract
    Lane 2 : Mouse lung extract
    Lane 3 : HeLa cells expressing human soluble RAGE

    Secondary
    anti-rabbit IgG alkaline phosphatase conjugate

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • RAW 264.7 cells were stained with anti-RAGE (1ug/million cells) for 1hr at 4 deg C followed by staining with anti-rabbit IgG-PE conjugate. Cells were analyzed by flow cytometry. Unstained cells or cells stained with secondary antibody alone are represented in the background
  • All lanes : Anti-RAGE antibody (ab37647) at 1/500 dilution

    Lane 1 : HUVEC whole cell lysate
    Lane 2 : EVC304 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 17,40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab37647 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200.

    Specimen tube 006 is negative control

    See Abreview

  • ICC/IF image of ab37647 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37647, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ab37647 at a 1/100 dilution staining RAGE in bovine endothelial cells by Immunocytochemistry/ Immunofluorescence. Fixed in PFA, permeabilized with Triton X-100. Blocked using 10% serum for 20 minutes at room temperature. Secondary used at 1/200 polyclonal Goat snti-rabbit conjugated to Alexa Fluor 568 (red). Nuclear staining (blue).

    See Abreview

Anti-RAGE antibody (ab37647)参考文献

This product has been referenced in:
  • de Seny D  et al. Apolipoprotein-A1 as a damage-associated molecular patterns protein in osteoarthritis: ex vivo and in vitro pro-inflammatory properties. PLoS One 10:e0122904 (2015). Human . Read more (PubMed: 25849372) »
  • Stensen MH  et al. Advanced glycation end products and their receptor contribute to ovarian ageing. Hum Reprod 29:125-34 (2014). Human . Read more (PubMed: 24256989) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain, temporal cortex)
Antigen retrieval step Other
Permeabilization Yes - hydrogen peroxide in methanol
Specification Brain, temporal cortex
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Annabelle L. Chambers

Verified customer

提交于 Oct 11 2016

Application Western blot
Sample Human Cell lysate - whole cell (Human gingival epithelial cells)
Gel Running Conditions Reduced Denaturing (4-15%)
Loading amount 20 µg
Specification Human gingival epithelial cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 21 2015

Application Western blot
Sample Mouse Tissue lysate - whole (gingival tissue)
Gel Running Conditions Reduced Denaturing (4-15% GRADIENT GEL)
Loading amount 20 µg
Specification gingival tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 01 2015

Thank you for your enquiry.

I can confirm, as stated on the datasheet, that the immunogen for ab37647 Anti-RAGE antibody is as follows:

KGAPKKPPQRLEWKLNTGRT

This is corresponding to amino acids 39-58 of Human RAGE
...

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With the two antibodies you have been looking at I would suggest the following antibodies should serve as suitable isotype controls:

Rabbit polyclonal to RAGE (ab37647), rabbit IgG, isotype control: Rabbit IgG- ChIP Grade (ab37415)
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (stromal cell)
Specification stromal cell
Fixative Formaldehyde
Permeabilization Yes - Saponin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 02 2013

Thank you for contacting us. The probability of cross-reactivity with BSA is less than 1%.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande de remplacemen...

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Merci d'avoir pour ce supplément d’informations.

Malheureusement nous n’avons pas d’autre lot en stock pour ab37647 et je crains que si je vous envoie en remplacement le même lot, que vous obtiendrez les mê...

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Thank you for contacting us.

We have not tried this antibody in functional assay using living cells so I am sorry we are unable to confirm how well this antibody will perform. There are two basic things to consider when using ab in vivo, the p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"