概述

  • 产品名称Anti-RAG2抗体
    参阅全部 RAG2 一抗
  • 描述
    兔多克隆抗体to RAG2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Pig
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 271 - 519 of Human RAG2 (UniProt ID: P55895).

  • 阳性对照
    • WB: MOLT4 whole cell lysate ICC/IF: Hela cell IHC-P: DLD1 Xenograft Others: 293T, A431, H1299, HeLa, HepG2 and Raji cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab95955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
  • 组织特异性Cells of the B- and T-lymphocyte lineages.
  • 疾病相关Defects in RAG2 are a cause of combined cellular and humoral immune defects with granulomas (CHIDG) [MIM:233650]. CHIDG is an immunodeficiency disease with granulomas in the skin, mucous membranes, and internal organs. Other characteristics include hypogammaglobulinemia, a diminished number of T and B cells, and sparse thymic tissue on ultrasonography.
    Defects in RAG2 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive (T(-)B(-)NK(+) SCID) [MIM:601457]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Defects in RAG2 are a cause of Omenn syndrome (OS) [MIM:603554]. OS is a severe immunodeficiency characterized by the presence of activated, anergic, oligoclonal T-cells, hypereosinophilia, and high IgE levels.
  • 序列相似性Belongs to the RAG2 family.
    Contains 1 PHD-type zinc finger.
  • 结构域The atypical PHD-type zinc finger recognizes and binds histone H3 trimethylated on 'Lys-4' (H3K4me3). The presence Tyr-445 instead of a carboxylate in classical PHD-type zinc fingers results in an enhanced binding to H3K4me3 in presence of dimethylated on 'Arg-2' (H3R2me2) rather than inhibited. The atypical PHD-type zinc finger also binds various phosphoinositides, such as phosphatidylinositol-3,4-bisphosphate binding (PtdIns(3,4)P2), phosphatidylinositol-3,5-bisphosphate binding (PtdIns(3,5)P2), phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol-3,4,5-trisphosphate binding (PtdIns(3,4,5)P3).
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • RAG 2 antibody
    • RAG-2 antibody
    • RAG2 antibody
    • RAG2_HUMAN antibody
    • Recombination activating gene 2 antibody
    • V(D)J recombination activating protein 2 antibody
    • V(D)J recombination-activating protein 2 antibody
    see all

Anti-RAG2 antibody 图像

  • Anti-RAG2 antibody (ab95955) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 59 kDa
  • ab95955, at a 1/200 dilution, staining RAG2 in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
    The image on the right was co-stained using Hoechst 33342.
  • ab95955, at a 1/100 dilution, staining RAG2 in paraffin embedded DLD1 Xenograft by Immunohistochemistry.

Anti-RAG2 antibody (ab95955)参考文献

This product has been referenced in:
  • Lu Y  et al. CDK4 deficiency promotes genomic instability and enhances Myc-driven lymphomagenesis. J Clin Invest 124:1672-84 (2014). WB ; Human . Read more (PubMed: 24614102) »

See 1 Publication for this product

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