Anti-Raf1 (phospho Y341)抗体(ab59223)

概述

  • 产品名称Anti-Raf1 (phospho Y341)抗体
    参阅全部 Raf1 一抗
  • 描述
    兔多克隆抗体to Raf1 (phospho Y341)
  • 特异性ab59223 detects endogenous levels of Raf1 only when phosphorylated at tyrosine 341.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Raf1 around the phosphorylation site of tyrosine 341 (S-Y-YP-W-E).

  • 阳性对照
    • Human pancreas tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab59223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500. Predicted molecular weight: 73 kDa.

靶标

  • 功能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
  • 组织特异性In skeletal muscle, isoform 1 is more abundant than isoform 2.
  • 疾病相关Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
    Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
  • 细胞定位Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c Raf antibody
    • C-raf antibody
    • C-Raf proto-oncogene, serine/threonine kinase antibody
    • CMD1NN antibody
    • Craf 1 transforming gene antibody
    • cRaf antibody
    • Craf1 transforming gene antibody
    • EC 2.7.11.1 antibody
    • kinase Raf1 antibody
    • Murine sarcoma 3611 oncogene 1 antibody
    • NS5 antibody
    • Oncogene MIL antibody
    • Oncogene RAF1 antibody
    • OTTHUMP00000160218 antibody
    • OTTHUMP00000207813 antibody
    • OTTHUMP00000209389 antibody
    • Protein kinase raf 1 antibody
    • Proto-oncogene c-RAF antibody
    • Raf 1 antibody
    • Raf 1 proto oncogene serine/threonine kinase antibody
    • RAF antibody
    • Raf proto oncogene serine/threonine protein kinase antibody
    • RAF proto-oncogene serine/threonine-protein kinase antibody
    • RAF-1 antibody
    • RAF1 antibody
    • RAF1_HUMAN antibody
    • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
    • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
    • v raf 1 murine leukemia viral oncogene homolog 1 antibody
    • v-raf murine sarcoma viral oncogene homolog 1 antibody
    • vraf1 murine leukemia viral oncogene homolog 1 antibody
    see all

Anti-Raf1 (phospho Y341) antibody 图像

  • All lanes : Anti-Raf1 (phospho Y341) antibody (ab59223) at 1/500 dilution

    Lane 1 : Jurkat cell extracts treated with paclitaxel
    Lane 2 : Jurkat cell extracts treated with paclitaxel with antigen-specific peptide


    Predicted band size : 73 kDa
  • ab59223 (1/50 dilution) staining Raf1 in human pancreas tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunising peptide.ab59223 (1/50 dilution) staining Raf1 in human pancreas tissue by Immunohistochemistry (Paraffin section); in the absence or presence of the immunising peptide.

Anti-Raf1 (phospho Y341) antibody (ab59223)参考文献

ab59223 has not yet been referenced specifically in any publications.

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