Anti-Radixin抗体[AF1H1] (ab85323)

概述

  • 产品名称
    Anti-Radixin抗体[AF1H1]
    参阅全部 Radixin 一抗
  • 描述
    小鼠单克隆抗体[AF1H1] to Radixin
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    His tagged recombinant fragment, corresponding to amino acids 1-310 of Human Radixin, from E.coli.

  • 阳性对照
    • A431, HeLa, HepG2, K562, 293T, PC12 and NIH3T3 cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 克隆
    单克隆
  • 克隆编号
    AF1H1
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ELISA 1/10000.

靶标

  • 功能
    Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
  • 疾病相关
    Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 序列相似性
    Contains 1 FERM domain.
  • 结构域
    The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
  • 翻译后修饰
    Phosphorylated by tyrosine-protein kinases.
  • 细胞定位
    Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CB567 antibody
    • CG12537 antibody
    • DFNB24 antibody
    • ESP10 antibody
    • Hh-induced MATH and BTB domain-containing protein antibody
    • HIB antibody
    • Moesin-B antibody
    • Protein roadkill antibody
    • RADI_HUMAN antibody
    • Radixin antibody
    • RDX antibody
    see all

Anti-Radixin antibody [AF1H1] 图像

  • All lanes : Anti-Radixin antibody [AF1H1] (ab85323) at 1/10000 dilution

    Lane 1 : A431 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : HepG2 cell lysates
    Lane 4 : K562 cell lysates
    Lane 5 : 293T cell lysates
    Lane 6 : PC12 cell lysates
    Lane 7 : NIH3T3 cell lysates


    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-Radixin antibody [AF1H1] (ab85323)参考文献

ab85323 has not yet been referenced specifically in any publications.

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I apologise for the delay, I contacted my lab colleagues to check whether this antibody has ever been tested in IP applications. They confirmed it was once used in the past with bad results, therefore we wouldn&rs...

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Thank you for contacting us.

Unfortunately, the exact concentration of the Anti-Radixin antibody [AF1H1] cannot be determined.

This monoclonal antibody isproduced by ammonium sulfate precipitation from ascites. Different lots of thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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