概述
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产品名称Anti-Radixin抗体
参阅全部 Radixin 一抗 -
描述兔多克隆抗体to Radixin
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Radixin.
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阳性对照
- This antibody gave a positive signal in the following lysates: Brain (Human) Tissue Lysate - adult normal tissue; HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab91312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 mg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
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疾病相关Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
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序列相似性Contains 1 FERM domain.
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结构域The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
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翻译后修饰Phosphorylated by tyrosine-protein kinases.
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细胞定位Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
- Information by UniProt
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数据库链接
- Entrez Gene: 517111 Cow
- Entrez Gene: 5962 Human
- Entrez Gene: 19684 Mouse
- Entrez Gene: 494457 Pig
- Entrez Gene: 315655 Rat
- Omim: 179410 Human
- SwissProt: Q32LP2 Cow
- SwissProt: P35241 Human
see all -
别名
- CB567 antibody
- CG12537 antibody
- DFNB24 antibody
see all
Anti-Radixin antibody 图像
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Western blot - Radixin antibody (ab91312)All lanes : Anti-Radixin antibody (ab91312) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes -
Immunocytochemistry/ Immunofluorescence - Radixin antibody (ab91312)ICC/IF image of ab91312 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91312 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Radixin antibody (ab91312)参考文献
ab91312 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"