概述

  • 产品名称Anti-Rad9抗体
    参阅全部 Rad9 一抗
  • 描述
    山羊多克隆抗体to Rad9
  • 经测试应用适用于: Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    C-QGPSPVLAEDSEGE

    , corresponding to C terminal amino acids 377-391 of Human Rad9

  • 阳性对照
    • A431 and Daudi cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 5 - 10 µg/ml. Used as a reporter to detect recombinant RAD9A
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 序列相似性Belongs to the rad9 family.
  • 翻译后修饰Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell cycle checkpoint control protein antibody
    • Cell cycle checkpoint control protein RAD9A antibody
    • DNA repair exonuclease rad9 homolog A antibody
    • hRAD 9 antibody
    • hRAD9 antibody
    • Rad 9 antibody
    • RAD 9A antibody
    • RAD9 (S pombe) homolog antibody
    • RAD9 homolog A antibody
    • RAD9 homolog antibody
    • Rad9a antibody
    • RAD9A_HUMAN antibody
    see all

Anti-Rad9 antibody 图像

Anti-Rad9 antibody (ab59022)参考文献

ab59022 has not yet been referenced specifically in any publications.

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