Anti-Rad9抗体[7B11] (ab117848)

概述

  • 产品名称Anti-Rad9抗体[7B11]
    参阅全部 Rad9 一抗
  • 描述
    小鼠单克隆抗体[7B11] to Rad9
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Protein expressed in 293T cells transfected with Human Rad9 expression vector

  • 阳性对照
    • HeLa, HT29 or Jurkat lysate HEK293T transfected lysate Human pancreas carcinoma, breast adenocarcinoma, colon, colon adenocarcinoma or lung carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 48% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from Mouse ascite fluid by affinity chromatography
  • 克隆单克隆
  • 克隆编号7B11
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 43 kDa.
IHC-P 1/50.

靶标

  • 功能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 序列相似性Belongs to the rad9 family.
  • 翻译后修饰Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell cycle checkpoint control protein antibody
    • Cell cycle checkpoint control protein RAD9A antibody
    • DNA repair exonuclease rad9 homolog A antibody
    • hRAD 9 antibody
    • hRAD9 antibody
    • Rad 9 antibody
    • RAD 9A antibody
    • RAD9 (S pombe) homolog antibody
    • RAD9 homolog A antibody
    • RAD9 homolog antibody
    • Rad9a antibody
    • RAD9A_HUMAN antibody
    see all

Anti-Rad9 antibody [7B11] 图像

  • All lanes : Anti-Rad9 antibody [7B11] (ab117848) at 1/2000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : HeLa lysate
    Lane 3 : HT29 lysate
    Lane 4 : A549 lysate
    Lane 5 : COS7 lysate
    Lane 6 : Jurkat lysate
    Lane 7 : MDCK lysate
    Lane 8 : PC12 lysate
    Lane 9 : MCF7 lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 43 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC204439)
  • ab117848, at a 1/50 dilution, staining Human Rad9 in breast adenocarcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.
  • ab117848, at a 1/50 dilution, staining Human Rad9 in colon, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.
  • ab117848, at a 1/50 dilution, staining Human Rad9 in colon adenocarcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.
  • ab117848, at a 1/50 dilution, staining Human Rad9 in lung carcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.
  • ab117848, at a 1/50 dilution, staining Human Rad9 in pancreas carcinoma, using Immunohistochemistry, formalin/PFA-fixed paraffin embedded tissue.

Anti-Rad9 antibody [7B11] (ab117848)参考文献

ab117848 has not yet been referenced specifically in any publications.

Product Wall

AB70810 DISCOUNT CODE: XXXX Expiration date: AB117848 DISCOUNT CODE: XXXX Expiration date: I am very pleased to hear you would like to accept our offer and test both ab70810 and ab117848 and in ChIP. Above are the code that I have created for each...

Read More

Thank you for contacting us. While we do not have a ChIP graded RAD9A antibody. It is highly likely that the products we do have may work in your experiment. Ideally, specific antibodies for ChIP should be affinity-purified. Polyclonal antibodies are...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"