- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-RAD54L2抗体
参阅全部 RAD54L2 一抗 -
描述兔多克隆抗体to RAD54L2
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宿主Rabbit
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经测试应用适用于: WB, IP, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster, Elephant
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免疫原
Synthetic peptide, corresponding to a region between residues 75 and 125 of human RAD54L2 (NP_055921.2)
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阳性对照
- HeLa whole cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab86063 was affinity purified using an epitope specific to RAD54L2 immobilized on solid support.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab86063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 163 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro.
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序列相似性Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
结构域Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to be important for the association with nuclear receptors.
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翻译后修饰Sumoylated.
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细胞定位Nucleus. Localizes in speckle-like nuclear compartments.
- Information by UniProt
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数据库链接
- Entrez Gene: 23132 Human
- Entrez Gene: 81000 Mouse
- Entrez Gene: 363135 Rat
- SwissProt: Q9Y4B4 Human
- SwissProt: Q99NG0 Mouse
- Unigene: 105399 Human
- Unigene: 246689 Mouse
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别名
- Androgen receptor interacting protein 4 antibody
- Androgen receptor-interacting protein 4 antibody
- Arip4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAD54L2 antibody (ab86063)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling RAD54L2 with ab86063 at 1/1200 (1µg/ml). Detection: DAB. -
Western blot - Anti-RAD54L2 antibody (ab86063)All lanes : Anti-RAD54L2 antibody (ab86063) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 163 kDa
Observed band size: 190 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunoprecipitation - Anti-RAD54L2 antibody (ab86063)Detection of RAD54L2 by Western Blot of Immunprecipitate.
ab86063 at 1µg/ml staining RAD54L2 in HeLa whole cell lysate immunoprecipitated using ab86063 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
文献
This product has been referenced in:
- Suzuki J et al. Xkr8 phospholipid scrambling complex in apoptotic phosphatidylserine exposure. Proc Natl Acad Sci U S A 113:9509-14 (2016). WB . Read more (PubMed: 27503893) »
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