Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within the internal amino acids 861-910 (NSLKPLSMSQ LKQWKHFSGD HLNLTDPFLE RITENVSFIF QNITTQATGT) of human RAD54B (NP_036547).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
ab83446 is purified by peptide affinity chromatography method.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent dilution. Titer in peptide based assay is 1/1562500.
The RAD52 epistasis group genes are involved in homologous recombination, and they are conserved from yeast to humans. RAD54B is involved in DNA repair and mitotic recombination.
DNA repair and recombination protein RAD54B antibody
EC 3.6.1.- antibody
Fibrinogen silencer binding protein antibody
Western blot - Anti-RAD54B antibody (ab83446)
Anti-RAD54B antibody (ab83446) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
90 kDa (
why is the actual band size different from the predicted?
Gel concentration: 12%
Immunocytochemistry/ Immunofluorescence - Anti-RAD54B antibody (ab83446) This image is courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab83446 (1/500) staining RAD54B in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"