Anti-RAD54B抗体(ab83446)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to RAD54B
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 861-910 (NSLKPLSMSQ LKQWKHFSGD HLNLTDPFLE RITENVSFIF QNITTQATGT) of human RAD54B (NP_036547).

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution. Titer in peptide based assay is 1/1562500.
ICC/IF 1/500.

靶标

  • 相关性
    The RAD52 epistasis group genes are involved in homologous recombination, and they are conserved from yeast to humans. RAD54B is involved in DNA repair and mitotic recombination.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • DNA repair and recombination protein RAD54B antibody
    • EC 3.6.1.- antibody
    • Fibrinogen silencer binding protein antibody
    • FSBP antibody
    • RAD54 homolog B antibody
    • RDH54 antibody
    see all

图片

  • Anti-RAD54B antibody (ab83446) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 103 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab83446 (1/500) staining RAD54B in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

文献

ab83446 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton x100
Username

Dr. Kirk Mcmanus

Verified customer

提交于 May 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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