Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within the internal amino acids 861-910 (NSLKPLSMSQ LKQWKHFSGD HLNLTDPFLE RITENVSFIF QNITTQATGT) of human RAD54B (NP_036547).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
ab83446 is purified by peptide affinity chromatography method.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent dilution. Titer in peptide based assay is 1/1562500.
Anti-RAD54B antibody 图像
Western blot - RAD54B antibody (ab83446)
Immunocytochemistry/ Immunofluorescence - RAD54B antibody (ab83446) This image is courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab83446 (1/500) staining RAD54B in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Anti-RAD54B antibody (ab83446)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"