Rabbit, Cow, Cat
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (RRSAAPSQLQ GNSFKKPKFI PPGRSNPGLN EEITKLNPDI KLFEGVAINN) of Human RAD54B (NP_036547).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 103 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
The RAD52 epistasis group genes are involved in homologous recombination, and they are conserved from yeast to humans. RAD54B is involved in DNA repair and mitotic recombination.
DNA repair and recombination protein RAD54B antibody
EC 3.6.1.- antibody
Fibrinogen silencer binding protein antibody
Western blot - Anti-RAD54B antibody (ab83311)
Anti-RAD54B antibody (ab83311) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
90 kDa (
why is the actual band size different from the predicted?
Gel concentration: 6-18%
Immunocytochemistry/ Immunofluorescence - Anti-RAD54B antibody (ab83311) Image part of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab83311 (1/200) staining RAD54B in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"