概述

  • 产品名称Anti-RAD54B抗体
    参阅全部 RAD54B 一抗
  • 描述
    兔多克隆抗体to RAD54B
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (RRSAAPSQLQ GNSFKKPKFI PPGRSNPGLN EEITKLNPDI KLFEGVAINN) of Human RAD54B (NP_036547).

  • 阳性对照
    • Jurkat cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab83311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 103 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200.

靶标

  • 相关性The RAD52 epistasis group genes are involved in homologous recombination, and they are conserved from yeast to humans. RAD54B is involved in DNA repair and mitotic recombination.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • DNA repair and recombination protein RAD54B antibody
    • EC 3.6.1.- antibody
    • Fibrinogen silencer binding protein antibody
    • FSBP antibody
    • RAD54 homolog B antibody
    see all

Anti-RAD54B antibody 图像

  • Anti-RAD54B antibody (ab83311) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 103 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab83311 (1/200) staining RAD54B in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

    See Abreview

Anti-RAD54B antibody (ab83311)参考文献

This product has been referenced in:
  • Sajesh BV  et al. Synthetic lethal targeting of superoxide dismutase 1 selectively kills RAD54B-deficient colorectal cancer cells. Genetics 195:757-67 (2013). Read more (PubMed: 24002644) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"