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Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Saccharomyces cerevisiae Rad53.
Our Abpromise guarantee covers the use of ab104232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 25817432|
|WB||1/2000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). Abcam recommends using milk as the blocking agent (4%)|
Michela Clerici, University of Milano-Biococca
Blocking: 4% milk for 1 hour
Phosphorylation of Rad53 induced by phleomycin treatment see PubmedID:152856
This image is courtesy of Ilaria Guerini, Gurdon Institute, United Kingdom