• 产品名称Anti-RAD52抗体
    参阅全部 RAD52 一抗
  • 描述
    兔多克隆抗体to RAD52
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human RAD52.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; MCF7; HeLa; HEk293; U2OS as well as HeLa Nuclear lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab103067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa). Abcam recommends using milk as the blocking agent (3%)
ICC/IF 1/200.

Use with paraformaldehyde-fixed cells.


  • 功能Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
  • 序列相似性Belongs to the RAD52 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA repair protein RAD52 antibody
    • DNA repair protein RAD52 antibody
    • DNA repair protein RAD52 homolog antibody
    • RAD 52 antibody
    • Rad52 antibody
    • RAD52 homolog (S. cerevisiae) antibody
    • RAD52 homolog (S. cerevisiae) antibody
    • RAD52 homolog antibody
    • RAD52_HUMAN antibody
    • Recombination protein RAD52 antibody
    • Recombination protein RAD52 antibody
    • Rhabdomyosarcoma antigen MU RMS 40.23 antibody
    see all

Anti-RAD52 antibody 图像

  • All lanes : Anti-RAD52 antibody (ab103067) at 1/250 dilution (Milk Blocking - 3%)

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Exposure time : 20 minutes
    Abcam recommends using milk as the blocking agent (3%). This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab103067 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • ab103067 (1/200) staining RAD52 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

Anti-RAD52 antibody (ab103067)参考文献

ab103067 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X100 in PBS

Dr. Kirk McManus

Verified customer

提交于 May 10 2013