概述

  • 产品名称Anti-RAD51AP1抗体
    参阅全部 RAD51AP1 一抗
  • 描述
    小鼠多克隆抗体to RAD51AP1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human RAD51AP1, amino acids 1-335 (NP_006470.1).

  • 阳性对照
    • Human colon tissue lysate, RAD51AP1 transfected 293T cell lysate, HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Functionally cooperates with PALB2 in promoting of D-loop formation by RAD51. Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.
  • 组织特异性Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Colocalizes with RAD51 to multiple nuclear foci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2510006L10Rik antibody
    • MGC156698 antibody
    • OTTMUSP00000028651 antibody
    • PIR51 antibody
    • R51A1_HUMAN antibody
    • RAB22 antibody
    • RAD51 associated protein 1 antibody
    • RAD51 interacting protein antibody
    • RAD51-associated protein 1 antibody
    • RAD51-interacting protein antibody
    • Rad51ap1 antibody
    see all

Anti-RAD51AP1 antibody 图像

  • Anti-RAD51AP1 antibody (ab88370) at 1 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RAD51AP1 antibody (ab88370) at 1 µg/ml

    Lane 1 : RAD51AP1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • ab88370 at 10µg/ml staining RAD51AP1 in HeLa cells by Immunofluorescence.

Anti-RAD51AP1 antibody (ab88370)参考文献

ab88370 has not yet been referenced specifically in any publications.

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