Anti-Rad51抗体- ChIP Grade (ab63798)

概述

  • 产品名称Anti-Rad51抗体- ChIP Grade
    参阅全部 Rad51 一抗
  • 描述
    兔多克隆抗体to Rad51 - ChIP Grade
  • 经测试应用适用于: WB, IP, ICC/IF, ChIPmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Recombinant Rad51 protein (Saccharomyces cerevisiae) purified.

  • 阳性对照
    • Extract of wild-type S.cervisiae strain.

性能

应用

Our Abpromise guarantee covers the use of ab63798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 43 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration. PubMed: 22343724

靶标

  • 功能Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • 组织特异性Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • 疾病相关Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • 序列相似性Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • 结构域The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • 翻译后修饰Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • 细胞定位Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt
  • 别名
    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Anti-Rad51 antibody - ChIP Grade (ab63798)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

I am very pleased to hear you would like to accept our offer and test ab63798 for ChIP. This code will give you 1 free primary antibody before the expiration date. Your move from one institute to another should not have an effect in this. As long as yo...

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Thank you for contacting us. The Anti-Rad51 antibody (ab63798) you are interested in has to our knowledge not been tested with ChIP as of yet. However, if you choose to purchase this antibody you would qualify for a testing discount, more information o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"