Anti-Rad51抗体[51RAD01] (ab1837)


  • 产品名称
    参阅全部 Rad51 一抗
  • 描述
    小鼠单克隆抗体[51RAD01] to Rad51
  • 经测试应用
    适用于: Flow Cyt, WB, IP, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    BALB/C mice were injected with recombinant Rad51 protein.

  • 阳性对照
    • Testis


  • 形式
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
  • 克隆编号
  • 同种型
  • 轻链类型
  • 研究领域


Our Abpromise guarantee covers the use of ab1837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/10. PMID 18663141
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
    • 组织特异性
      Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
    • 疾病相关
      Breast cancer
      Mirror movements 2
      Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
    • 序列相似性
      Belongs to the RecA family. RAD51 subfamily.
      Contains 1 HhH domain.
    • 结构域
      The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
    • 翻译后修饰
      Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
      Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
    • 细胞定位
      Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BRCA1/BRCA2 containing complex, subunit 5 antibody
      • BRCC 5 antibody
      • BRCC5 antibody
      • DNA repair protein RAD51 homolog 1 antibody
      • DNA repair protein rhp51 antibody
      • FANCR antibody
      • hRAD51 antibody
      • HsRAD51 antibody
      • HsT16930 antibody
      • MRMV2 antibody
      • Rad 51 antibody
      • RAD51 antibody
      • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
      • RAD51 homolog A antibody
      • RAD51 homolog antibody
      • RAD51 recombinase antibody
      • RAD51, S. cerevisiae, homolog of antibody
      • RAD51_HUMAN antibody
      • RAD51A antibody
      • RECA antibody
      • RecA like protein antibody
      • RecA, E. coli, homolog of antibody
      • Recombination protein A antibody
      see all

    Anti-Rad51 antibody [51RAD01] 图像

    • Overlay histogram showing HeLa cells stained with ab1837 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1837, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was goat anti-mouse DyLight® 488 goat (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunofluorescence analysis of wild-type (+/+) and Hormad1-/- (-/-) murine zygotene spermatocytes, staining Rad51 with ab1837.

    Anti-Rad51 antibody [51RAD01] (ab1837)参考文献

    This product has been referenced in:
    • Carofiglio F  et al. SPO11-independent DNA repair foci and their role in meiotic silencing. PLoS Genet 9:e1003538 (2013). ICC/IF . Read more (PubMed: 23754961) »
    • Somaiah N  et al. The relationship between homologous recombination repair and the sensitivity of human epidermis to the size of daily doses over a 5-week course of breast radiotherapy. Clin Cancer Res 18:5479-88 (2012). ICC/IF ; Human . Read more (PubMed: 22855580) »

    See all 11 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Planarian Cell (whole animal IF)
    Yes - tritonX-100
    whole animal IF
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

    Abcam user community

    Verified customer

    提交于 Jul 20 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
    Mouse Tissue sections (mouse testes)
    mouse testes
    4% PFA

    Abcam user community

    Verified customer

    提交于 May 29 2013

    That is no problem at all. I am glad to have been of some help. Please do let me know how you get on.

    Thank you for your email. I am sorry i was not able to get back to you yesterday.

    I can confirm we have had no other reports of concerns with the lot of the antibody you have been using. I have therefore arranged for a new vial of this lot to ...

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    Thank you for your reply and for letting me know how you have been getting on.

    I am sorry that my suggestions have not improved the results observed. As you say, especially with the positive results with the rabbit monoclonal ab133534, it may ...

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    Thank you for your reply and confirming those details.

    I think it would be worthwhile to try the different blocking conditions I suggested. Sometimes different antibodies can react quite differently using different blocking conditions. This ca...

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    Thank you for taking time to contact us and report the problems you have encountered in using the Anti-Rad51 antibody [51RAD01] (ab1837). I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will en...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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    Thank you for contacting us.

    Ice-cold methanol for 5-10 minutes would be ideal.

    I would suggest not storing the samples however if it is unavoidable then storing cells after fixation on chamber slides would be the option to go for. Read More

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    We haven't received any other complaint for this lot so I can reassure you that the quality of this antibody is absolutely fine. I have r...

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